FIG. 4.

TCR Vβ repertoire analysis of superantigen-stimulated PBMC by the Immunoscope approach. (A) Quantitative Vβ repertoire determined by real-time PCR analysis on day 0 (D0) and day 14 (D14) following stimulation with SElV or SElU2. The x axis indicates Vβ families and the y axis their relative frequency of usage. Selective Vβ expansion was considered to occur when the D14/D0 Vβ frequency ratio was >3. (B) Immunoscope profiles of the fluorescent Vβ-Cβ runoff products obtained with 14-day-stimulated mononuclear cells. The x axis indicates the CDR3 length. Only profiles corresponding to Vβ expansion are shown.