FIG. 1.

Deletion and complementation of the pkaR gene. A. The wild-type locus for pkaR. B. The majority of the open reading frame (black arrow) for pkaR in A. fumigatus was deleted and replaced with the hygromycin resistance gene (Hyg R) by homologous recombination to produce ΔpkaR. C. A wild-type gene, flanked by the phleomycin resistance gene (phleo R), was integrated into the right flanking DNA in the deletion strain to complement the mutation, producing pkaR C′. D. Genomic DNA from each member of the isogenic set was cut with PstI and probed with a piece of the left arm (black box marked probe) to detect the wild-type (4.2 kb), deletion (2.7 kb), and recombinant (6.1 kb) bands. P, PstI; H, HindIII; Sm, SmaI; Sa, SalI; Xb, XbaI; Xh, XhoI.