TABLE 1.

PKA activity in the ΔpkaR mutant is not regulated by cAMP^a

Genotype	PKA activity (U mg−1 of protein)
	Basal	+cAMP
Wild type (H237)	0.24 ± 0.11	0.65 ± 0.18b
ΔpkaR	0.41 ± 0.22	0.40 ± 0.18
pkaR C′	0.27 ± 0.15	0.51 ± 0.15b

^aPKA activity was measured on cell-free hyphal extracts before (basal) and after (+cAMP) the addition of cAMP to a final concentration of 1 μM.

^bThe +cAMP value significantly differs from the basal level, with a P value of <0.01.