Immunoblot analysis of streptococci with anti-PsaA antiserum. Soluble cell extracts of streptococci were subjected to Western blotting with anti-PsaA antiserum. (A) Lanes: 1, S. ratti; 2, S. orisratti; 3, S. vestibularis; 4, S. oralis; 5, S. mitis reference strain; 6, rPsaA. (B) Lanes: 1, S. mitis; 2, S. criceti; 3, S. pneumoniae 491/00; 4, rPsaA. Molecular mass markers in kilodaltons are indicated on the right.