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. 2006 Aug;74(8):4735–4743. doi: 10.1128/IAI.00165-06

TABLE 1.

Primer sequences used in RT-PCR for cytokine mRNA expression

Cytokine Primer sequence (5′-3′) Product size (bp) Reference
IL-2 ATGTACAGGATGCAACTCCTGTCTT 457 8a
GTTAGTGTTGAGATGATGCTTTGAC
IL-4 AGCCTCACAGAGCAGAAGACTCTG 99 37a
CAGCCCTGCAGAAGGTTTCCTTCTC
IL-5 GCTTCTGCATTTGAGTTTGCTAGCT 293 8a
TGGCCGTCAATGTATTTCTTTATTAAG
IL-10 CTTCGAGATCTCCGAGATGCCTTC 121 37a
GGATCATCTCAGACAAGGCTTGGC
IL-12 CCTGCTGGTGGCTGACGACAAT 311 15a
CTTCAGCTGCAAGTTCTTGGGT
IFN-γ GGACCCATATGTAAAAGAAGCAGA 121 37a
TGTCACTCTCCTCTTTCCAATTCT
TNF-α AGAGGGAAGAGTCCCCCAGGA 81 37a
TCAGCTTGAGGGTTTGCTACAA
GAPDH CGGATTTGGTCGTATTGG 203 36a
AGATGGTGATGGGATTTC