FIG. 6.

Formation of the μ1/μ1C ds bonds during the course of infection. T1L reovirus-infected cells were harvested at either 24, 48, 72, or 96 h postinfection. 50 mM IAM was added immediately upon resuspension of the infected cells in homogenization buffer. After purification, virions from each time point were mixed with nonreducing sample buffer (NR) and disrupted by boiling. The viral proteins were then resolved on a mini-sized SDS-8% PAGE gel. The amount of ds-bonded μ1/μ1C (% ds μ1/μ1C) relative to the total μ1, μ1C, and ds-bonded μ1/μ1C in each preparation was determined by densitometry of the Coomassie-stained gel. Immediately before harvesting, aliquots were taken from each time point and the proportion of dead/dying cells (% dead cells) was determined by trypan blue staining.