TABLE 5.
Conjugate | γDPGA (mol)/rPA (mol) | Dose (μg/mouse) | Anti-γDPGA (EU)
|
Anti-rPA (μg/ml)
|
||
---|---|---|---|---|---|---|
2nd injection | 3rd injection | 2nd injection | 3rd injection | |||
rPA/Cys-Gly3- γDPGA10 | 22 | 0.31 | 490 | 3,500 | 0.3 | 9 |
0.63 | 980 | 4,900 | 0.6 | 37 | ||
1.25 | 2,300 | 6,200 | 2 | 120 | ||
2.5 | 2,200 | 5,800 | 2 | 200 | ||
20 | NTb | 3,700 | NT | 440 |
Five- to 6-week-old NIH general-purpose mice (n = 10) were injected subcutaneously with 2.5 μg of γDPGA as a conjugate 2 weeks apart and exsanguinated 7 days after the second or third injection. IgG anti-γDPGA and anti-rPA were measured by ELISA, and the results are expressed as the geometric means.
NT, not tested.