TABLE 5.

Dose/immunogenicity relationships of conjugates prepared with a 10-mer of γDPGA bound to rPA^a

Conjugate	γDPGA (mol)/rPA (mol)	Dose (μg/mouse)	Anti-γDPGA (EU)		Anti-rPA (μg/ml)
			2nd injection	3rd injection	2nd injection	3rd injection
rPA/Cys-Gly3- γDPGA10	22	0.31	490	3,500	0.3	9
	0.63	980	4,900	0.6	37
		1.25	2,300	6,200	2	120
		2.5	2,200	5,800	2	200
		20	NTb	3,700	NT	440

^aFive- to 6-week-old NIH general-purpose mice (n = 10) were injected subcutaneously with 2.5 μg of γDPGA as a conjugate 2 weeks apart and exsanguinated 7 days after the second or third injection. IgG anti-γDPGA and anti-rPA were measured by ELISA, and the results are expressed as the geometric means.

^bNT, not tested.