FIG. 7.

Cell invasion (A) and colonization (B) of mutant expressing nonglycosylated Cj1496c from plasmid and chromosome. (A) Results of an INT-407 cell invasion assay. Values are given relative to the invasion of the parent strain DRH212, which was set at 100%. The experiment was performed in triplicate and repeated on at least three separate occasions. Error bars indicate standard deviations. (B) Colonization of 1-day-old chicks. One-day-old chicks were inoculated with 1 × 10⁴ CFU bacteria. The number of bacteria present in the ceca at 7 days postinfection is reported as the number of CFU per gram cecal content. Each closed circle represents the number of bacteria obtained from an individual chick. The median of bacterial concentration recovered is represented by a bar.