FIG. 4.

Expression of the CT813 protein by various chlamydial species. (A) HeLa cells infected with C. trachomatis (serovars A, B, Ba, C, D, E, F, G, I, K, L1, and L2 as well as the mouse biovar MoPn), C. caviae (GPIC), C. psittacis (6BC), and C. pneumoniae (AR39) as indicated at the top of each panel were processed ∼30 to 50 h after infection for immunofluorescence staining with the mouse anti-CT813 (white in single color and red in tricolor) and rabbit anti-CT395 (green) antibodies plus Hoechst (blue). (B) The MoPn-infected sample was also stained with a mouse anti-TC0199 (the homologue of the CT813 protein in MoPn) antibody (red). The rest of the staining was the same as that shown in panel A. Note that anti-CT813 positively labeled the inclusion membrane in HeLa cells infected with all C. trachomatis serovars but not other strains. Although anti-CT813 failed to detect the MoPn inclusion membrane, the antibody raised with the homologue TC0199 did.