FIG. 1.

K⁺ depletion inhibits HRV2 internalization. HeLa-H1 cells seeded on coverslips were subjected to hypotonic shock for 5 min, washed, and incubated with HRV2 in K⁺-free medium for 20 min at 34°C. Control cells were treated with the same buffers containing 10 mM K⁺. Cells were fixed, and HRV2 was visualized by using the anti-HRV2 monoclonal antibody 8F5. Preparations were photographed by using a confocal microscope. Noninfected cells did not show any staining with 8F5. Bar, 10 μm.