FIG. 5.

HRV2 internalization is blocked by transient overexpression of Amph-SH3. HeLa-H1 cells seeded on coverslips were transfected with an expression plasmid encoding myc-tagged Amph-SH3. Two days after transfection, they were incubated with HRV2, fixed, and permeabilized. HRV2 and Amph-SH3 were detected by using anti-HRV2 monoclonal antibody 8F5 and anti-myc antiserum, respectively, followed by fluorescent conjugates. Cells were examined and photographed by using a confocal microscope. In cells overexpressing Amph-SH3, HRV2 does not accumulate in the perinuclear compartment or in the cytoplasm but remains attached to the plasma membrane. Bar, 10 μM.