FIG. 3.

Immunogold detection of HCV nonstructural proteins on the membranous web in replicon cells. (a) 9-13 replicon cells were labeled with NS5A-specific MAb 11H (7; kindly provided by Jan Albert Hellings, Organon Teknika B.V., Boxtel, The Netherlands). Gold particles accumulated on a membranous structure (surrounded by arrowheads). In addition, some label was found on the ER (arrows). Bar, 500 nm. (b) Identically treated naive Huh-7 cells were virtually devoid of gold particles. Bar, 500 nm. (c and d) At higher magnification, 9-13 replicon cells labeled with NS5A-specific MAb 11H (c) and NS3-specific MAb 1B6 (d). Both antigens are found almost exclusively on the membranous web. Bar, 200 nm.