FIG. 2.
Inhibition of gp120 and MAb binding by AD101 and SCH-C. (a) Binding of gp120JR-FL-CD4-IgG2 to L1.2 cells expressing the CCR5 coreceptor was measured in the presence of different concentrations of AD101 (black squares) or SCH-C (white circles). The percent inhibition of gp120-CCR5 binding is defined as (MFI with inhibitor/MFI without inhibitor) × 100% and is plotted as a function of AD101 or SCH-C concentration. (b) Alternatively, binding of anti-CCR5 MAbs to L1.2 cells was measured in the presence of 100 nM AD101 (gray bars) or SCH-C (white bars). The percent inhibition of MAb binding is defined as (MFI with inhibitor/MFI without inhibitor) × 100%. All values are means ± SD of three independent experiments.