TABLE 2.
Plasmids
| Plasmid(s) | Description | Source or reference |
|---|---|---|
| pHL167-1 | EcoRI fragment containing Tf1 Gag sequence | 38 |
| pHL449-1 | neoA1-marked version of Tf1 in a ura4 selectable plasmid | 34 |
| pHL490-80 | Tf1-neoAI with frameshift in PR | 6 |
| pHL476-3 | Tf1-neoAI with frameshift in IN | 34 |
| pFL20 | S. pombe vector with autonomous replicating sequence and stabilization fragment | 42 |
| pHL891-19 | neoA1-marked Tf1 in a ura4 selectable plasmid; XhoI-less neo without intron; SpeI site just 3′ downstream of hairpin after Tf1 primer binding site | 40 |
| pHL1260 and pHL1261 | Tf1-neoA1 with deletion of amino acids 2 to 11, (ΔA) | This study |
| pHL1262 and pHL1263 | Tf1-neoA1 with deletion of amino acids 64 to 73 (ΔB) | This study |
| pHL1264 and pHL1265 | Tf1-neoA1 with deletion of amino acids 165 to 174 (ΔC) | This study |
| pHL1258 and pHL1259 | Tf1-neoA1 with deletion of amino acids 215 to 224 (ΔD) | This study |