Figure 3.

Two-color FISH analysis of tail fibroblasts prepared from a 4-week-old double-Tc mouse. (A) Metaphase spreads were hybridized to 14/22cen (red, arrow) and 2cen (green, arrowhead) probes to detect hCF(SC20) and hCF(2-W23), respectively. A photomicrograph of a representative spread is shown. A magnified (×2) image of the hCFs is provided in the inset. (B) Interphase nuclei were hybridized to 14/22cen (red) and 2cen (green) probes. Nuclei containing both signals and a red signal only are indicated with stars and arrowheads, respectively.