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. 2003 May;77(9):5241–5252. doi: 10.1128/JVI.77.9.5241-5252.2003

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FIG. 5. — Localization of c-Jun domains important for interaction with T-Ag. (A) Whole-cell extracts prepared from HJC-15b cells (0.25 mg) were incubated with either GST alone (lane 2), GST-c-Jun (lane 3), or deletion mutants of c-Jun fused to GST (lanes 4 to 8) (2 μg each) which were immobilized on glutathione-Sepharose beads. The Sepharose beads were washed extensively, and bound proteins were resolved by SDS-10% PAGE, transferred onto nitrocellulose membrane, and detected with an anti-SV40 T-Ag antibody (Ab-2 416). (B) SDS-10% PAGE analysis of GST, GST-T-Ag, and T-Ag deletion mutants. In lane 1, 10 μg of whole-cell extract from HJC-15b cells was loaded as positive control. (C) Summary of the results obtained from mapping assays. A schematic representation of c-Jun is shown at the top (not to scale). The abilities of c-Jun and its deletion mutants to interact with T-Ag are shown on the right. +++, very strong interaction; ++, strong interaction; +, reduced or weak interaction; −, no interaction.

FIG. 5. — Localization of c-Jun domains important for interaction with T-Ag. (A) Whole-cell extracts prepared from HJC-15b cells (0.25 mg) were incubated with either GST alone (lane 2), GST-c-Jun (lane 3), or deletion mutants of c-Jun fused to GST (lanes 4 to 8) (2 μg each) which were immobilized on glutathione-Sepharose beads. The Sepharose beads were washed extensively, and bound proteins were resolved by SDS-10% PAGE, transferred onto nitrocellulose membrane, and detected with an anti-SV40 T-Ag antibody (Ab-2 416). (B) SDS-10% PAGE analysis of GST, GST-T-Ag, and T-Ag deletion mutants. In lane 1, 10 μg of whole-cell extract from HJC-15b cells was loaded as positive control. (C) Summary of the results obtained from mapping assays. A schematic representation of c-Jun is shown at the top (not to scale). The abilities of c-Jun and its deletion mutants to interact with T-Ag are shown on the right. +++, very strong interaction; ++, strong interaction; +, reduced or weak interaction; −, no interaction.