FIG. 7.

Proliferative responses against MV H antigen. Splenocytes from cotton rats immunized i.n. with three doses of CVD 908-htrA and CVD 1208 alone or carrying MV DNA vaccine plasmids, and i.m. with plasmids pGA3-mH or pMSIN-H, as described in Fig. 6, were incubated in vitro in the presence of MV H antigen or bovine serum albumin (BSA) as control. Responses were studied 4 months after primary immunization. Bars indicate peak responses within the range of concentrations tested (0.01 to 10 μg/ml for BSA and 1/20 to 1/1/80,000 for MV H antigen) and are expressed as the stimulation index ± the SE of triplicate wells. ✽, Significant responses (P < 0.05) compared to control groups. The results shown are representative of two separate experiments.