FIG. 4.

An LT-IS patch at a distal location does not enhance responses to injected influenza virus vaccine. Mice were immunized on days 0 and 14 by i.d injection with 5 μg of trivalent influenza virus vaccine at the dorsal caudal region, and LT (5, 25, or 50 μg) or PBS (no LT) was loaded onto a patch and applied on the skin over the site of injection (A) or at the dorsal cervical region (B). Serum collected 2 weeks after the second immunization was measured by ELISA for B/Johannesburg-specific IgG titers. Individual titers are displayed (open circles) with the corresponding geometric mean (bar) for each group.