Figure 4.

The ω protein does not displace σ^ARNAP from the Pcop1-Pcop2 DNA. [α³²P]-Pcop1-Pcop2 DNA (2 nM) incubated with 60 nM ω protein (lane 2) or 60 nM σ^ARNAP (lane 3) or with both the ω (60 nM) and σ^ARNAP (60 nM) (lane 4) and the complexes analyzed by ndPAGE. The free DNA, ω, and σ^ARNAP complexes RNAP (I) and (II) are indicated. + and − indicate the presence or absence of the denoted protein, respectively.