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Schematic representations of plasmid DNA constructs. Plasmids pT7HB2.7, p1421, p123, p201, p124, and p202 direct the synthesis of wt SML (SML), ngSML, wt S (S), ngS, wt L (L), and ngL, respectively. Start codons (ATG) for S, M, and L open reading frames and start codons mutated to threonine codons (ACG) are indicated. The thick bars represent wt and unglycosylated envelope proteins. NG and 0G indicate N and O glycosylation sites, respectively.
