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. 2003 May;77(9):5084–5097. doi: 10.1128/JVI.77.9.5084-5097.2003

FIG. 1.

FIG. 1.

PCR strategy for cloning a region of divergent locus B (DL-B) of RFHVMm. The order and orientation of the genes within the DL-B region of KSHV flanking the DNA polymerase genes are shown (the size of each ORF is indicated in base pairs). The positions of the DNA polymerase-specific PCR primers QIRQB and DRIPA and the PCR product obtained by partial inverse amplification of the ORF 10 flanking region of RFHVMm are indicated. The positions of the DMGLB and RHFGA primer pools used for CODEHOP amplification of a region of the RFHVMm TS gene are also shown. Finally, the 3.6-kb PCR fragment of RFHVMm obtained by long-range PCR amplification between the ORF 10-specific primer F6565 and the TS-specific primer R20432 is indicated.