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CODEHOP PCR amplification of a region of the RFHVMm vTS gene with a thermal gradient. PCR amplification of DNA from the retroperitoneal fibromatosis tumor from MmuYN91 was performed with CODEHOPs derived from two conserved TS motifs, RHFGA and DMGLB. A gradient of annealing temperatures ranging from 55 to 70oC was used. The expected 284-bp fragment is indicated. Lane temperatures: 1, 70°C; 2, 68.9°C; 3, 67.1°C; 4, 64.3°C; 5, 60.5°C; 6, 57.9°C; 7, 56.1°C; 8, 55.0°C.
