TABLE 2.

Putative RFHVMn and RFHVMm gene products and comparisons with homologs present in other gammaherpesviruses

RFHV ORF designationa	Location RFHVMm/ RFHVMnb	Orien- tation	ORF size in amino acids (% identity with the RFHVMm ORF)						Possible function
			RFHVMm	RFHVMn	KSHV	RRV	HVS	EBV
ORF 8 (3′ end)	1-331/1-331	+	110c	110c (90)	847 (64)	829 (53)	808 (32)	857 (36)	Glycoprotein B
ORF 9	373-3411/373-3411	+	1,013	1,013 (87)	1,012 (74)	1,014 (67)	1,009 (60)	1,015 (53)	DNA polymerase
ORF 10	3514-4746/3528-4760	+	411	411 (75)	418 (46)	416 (32)	407 (23)	422d (12)	Unknown
ORF 11	NPe		NP	NP	407	409	405	429f	Unknown
RF2	5624-4998/5632-5006	−	209	209 (74)	204 (35)	208 (19)	NP	NP	IL-6 homolog
ORF02	6252-5629/6260-5637	−	208	208 (84)	210 (52)	188 (41)	187 (45)	NP	Dihydrofolate reductase
RF3/ORF 12g	7059-6360/7059-6363	−	233	232 (85)	333 (40)	NP	169 (18)	NP	Modulator of immune response
ORF 70 (3′ end)	7739-7229/7756-7246	−	170c	170c (86)	337 (74)	333 (69)	294 (66)	NP	Thymidylate synthase

^aDerived from the HVS and KSHV ORF nomenclature, with non-HVS ORFs labeled with the prefix RF.

^bPosition within genomic DNA fragments of RFHVMm (7,739 bp) and RFHVMn (7,756 bp) extending from within ORF 8 to within the vTS gene assembled from the partial sequence of ORF 8 and complete sequence of ORF 9 (RFHVMm, AF005479; RFHVMn, AF204166) and the partial sequence of ORF 70 and the complete sequences of ORF 10, RF2, ORF 02, and RF3 (this study).

^cPartial sequence of the 3′ end.

^dLF1 gene present in the EBV Raji insertion.

^eNP, not present.

^fLF2 gene present in the EBV Raji insertion.

^gORF K3 in KSHV was not recognized as homologous to ORF 12 in HVS and thus was given a K designation in the original KSHV nomenclature (37). For this reason, the RFHV ORF was given the RF designation even though it is homologous to HVS ORF 12.