Identification of the PrV UL11 protein. Purified PrV-Ka, PrV-ΔUL11, and PrV-ΔUL11R virions were analyzed by Western blotting using monospecific antisera against the UL11, US3, UL49, and UL37 proteins as well as gM or monoclonal antibodies against gB, gE, and gC. Proteins were separated on an SDS-15% polyacrylamide gel for detection of the PrV UL11 gene product, and SDS-10% polyacrylamide gels were used for detection of the other tegument or envelope proteins. Locations of molecular mass markers are shown on the left.