FIG. 5.

Growth properties of PrV-ΔUL11. (A) Analysis of plaque size. RK13 (dark bars) or RK13-UL11 cells (light bars) were infected with PrV-Ka, PrV-ΔUL11, or PrV-ΔUL11R under plaque assay conditions, and plaque diameters were measured microscopically at 48 h p.i. The mean plaque size of PrV-Ka was set at 100%, and relative plaque sizes as well as standard deviations were calculated from three independent experiments. (B) One-step growth kinetics. RK13 cells were infected with PrV-Ka, PrV-ΔUL11, or PrV-ΔUL11R at an MOI of 5 and harvested at 0, 4, 8, 12, 24, and 36 h p.i. Total virus titers and standard deviations for three independent experiments are shown.