FIG. 3.

Replication and transcription in vivo of viral RNPs containing wild-type or mutant PB2 subunits. The accumulation of mRNA and cRNA was determined by RNase protection assay of polyadenylated and nonpolyadenylated RNA, respectively, derived from reconstitution experiments in which vRNA-like template was provided in vivo. Likewise, vRNA accumulation was determined from reconstitution experiments in which cRNA-like template was provided in vivo. Control (CTRL) experiments lacked PB2-encoding plasmid in the reconstitution. P, probe used for RNase protection; MW, size markers (nucleotides).