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. 2000 Jan 18;97(2):734–739. doi: 10.1073/pnas.97.2.734

Figure 2.

Figure 2

Effect of B16M-CM on adhesion of B16M cells to HSE. Cultured HSE cells were incubated in the presence of B16M-CM for 10 h. (A) Percent of adhering B16M cells to HSE incubated with B16M-CM in the presence of ICEi (10 μM) or anti-IL-18 (10 μg/ml). (B) Level of IL-1β in HSE supernatants before adhesion assay. (C) Level of TNF-α in HSE supernatants before adhesion assay. After the removal of the HSE supernatants, the adhesion cell assay was performed as described in Materials and Methods. Data represent the means ± SD of four separate experiments performed by using four different preparations of HSE cells, each in six replicates (n = 24). ∗, P < 0.01 for the percentage of B16M cells adhering to B16M-CM-treated HSE and of IL-1β and TNF-α production with respect to the basal medium. The Student's two-tailed, unpaired t test was used.