FIG. 5.

Extension from PPT62 at a nick or a gap followed by HET RNA. 5′ end-labeled PPT62 without a downstream oligonucleotide (none; lanes 1 to 4 and 9 to 12) or with downstream HET (lanes 5 to 8 and 13 to 16) was annealed to template 5 (lanes 1 to 8) or template 5B (lanes 9 to 16) to create a nick or a 5-base gap following the 3′ end of PPT62. The enzyme used for each set of extensions is indicated at the left. Experiments were performed and analyzed as described in the legend to Fig. 2. The positions of unextended PPT62 are indicated at right.