Uptake of AIB by R. leguminosarum exposed to different concentrations of various osmolytes. A. AIB uptake in cells exposed to increasing concentrations of sucrose (circles), NaCl (triangles), or mannitol (squares) immediately before assay. B. AIB uptake in cells exposed to 200 mM of the compound indicated on the x axis immediately before assay. Abbreviations: Suc, sucrose; Man, mannitol; Gly, glycerol; Glc, glucose; PEG 200, polyethylene glycol 200. Results are the mean uptake per minute over 4 minutes of at least three independent replicates shown plus and minus the standard error of the mean.