FIG. 3.

Transcriptional activities of various misR::lacZ promoter fusions. The schematic diagram of the misRS operon shows the locations of primers used to generate the promoter fragment. The cloned region with respect to the transcriptional start site (+1) is shown in parentheses. The dashed line indicates the 33-bp deleted region within the promoter in strain YT0324. β-Galactosidase activities of strains grown in GC broth to mid-log phase are expressed as Miller units ± standard deviation corresponding to mean values of triplicate measurements. The activity of a promoterless lacZ construct (strain 263) is 57.4 ± 1.7 Miller units.