FIG. 6.

Transcription of misR (A) and MisR protein production (B) are reduced in the misS mutant. (A) β-Galactosidase activities of misR::lacZ fusions in various genetic backgrounds. The misRS genotypes are indicated below the strains. Strain YT263 is the promoterless negative control. (B) Western blotting and probing with polyclonal anti-MisR antisera were performed on whole-cell lysates of the wild-type strain, the misR mutant, and the misS mutant. Equal cell numbers (1 × 10⁸ cells) were loaded in each sample.