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. 2006 Jul;188(14):5308–5314. doi: 10.1128/JB.00187-06

FIG. 3.

FIG. 3.

Characterization of the MotX mutants. (A) VPG plates containing 0.25% agar, kanamycin, and various concentrations of DTT were inoculated with fresh colonies of motX mutant cells (NMB94) expressing the indicated proteins from plasmids and incubated at 30°C for 4 h. (B) Whole-cell lysates of the NMB94 strain expressing the indicated proteins from plasmids were prepared and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting as described previously (35) using anti-MotX antibodies (MotXB0080) in the absence (−) and the presence (+) of 2-mercaptoethanol (2ME). The anti-MotX antibody was raised against purified MotX produced from pKJ402, which encoded C-terminally histidine-tagged MotX.