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Biotin maleimide labeling of MotX cysteine residues. NMB94 harboring pMO401 (MotX wild type) was treated with biotin maleimide (BM) and DTT or CuCl2. The immunoprecipitates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes. Biotinylated MotX was detected with streptavidin-conjugated horseradish peroxidase and chemiluminescence (A) or immunoblotting using anti-MotX antibodies (B).
