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. 2006 Jul;188(14):5308–5314. doi: 10.1128/JB.00187-06

TABLE 1.

Bacterial strains and plasmids

Strain or plasmid Genotype or descriptiona Source or reference
V. alginolyticus strains
    VIO5 VIK4 laf (Rifr Pof+ Laf) 28
    VIO542 VIO5 motY (Rifr Laf Mot) 28
    NMB94 VIO5 motX (Rifr Laf Mot) 26
    GRF2 VIO5 ΔmotY (Rifr Laf Mot) This study
E. coli strains
    JM109 recA1 endA1, gyrA96 thi hsdR17 relA1 supE44 λ Δ(lac-proAB) FtraD36 proAB lacIq ΔM15 33
    BL21(DE3) Stratagene, San Diego, CA
Plasmids
    pSU38 kan (Kmr) PlaclacZa 4
    pSU41 kan (Kmr) PlaclacZa 4
    pIO2 pSU21; 20-kb SalI fragment (motY+) 28
    pIO6 pSU38; 1.0-kb HindIII-XbaI fragment (motY+) 28
    pRK101 pIO6; motY (C147Y) This study
    pRK102 pIO6; motY (R262Q) This study
    pRK103 pIO6; motY (C25Y; −3S of signal sequence to N) This study
    pRK201 pSU38; 1.0-kb HindIII-XbaI fragment (motY C25S) This study
    pRK202 pSU38; 1.0-kb HindIII-XbaI fragment (motY C147S) This study
    pRK203 pSU38; 1.0-kb HindIII-XbaI fragment (motY C25S/C147S) This study
    pKS202 pSU41; 1.0-kb EcoRI-XbaI fragment (6His-motY) This study
    pMO401 pSU41; 700-bp ClaI-BamHI fragment (motX+) 26
    pMO404 pSU41; 700-bp ClaI-BamHI fragment (motX C35S) This study
    pMO405 pSU41; 700-bp ClaI-BamHI fragment (motX C66S) This study
    pMO413 pSU41; 700-bp ClaI-BamHI fragment (motX C35S/C66S) This study
a

Rifr, rifampin resistant; Kmr, kanamycin resistant; Cmr, chloramphenicol resistant; Plac, lac promoter; Pof+, normal polar-flagellar formation; Laf, defective in lateral-flagellar formation.