TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or descriptiona | Source or reference |
|---|---|---|
| V. alginolyticus strains | ||
| VIO5 | VIK4 laf (Rifr Pof+ Laf−) | 28 |
| VIO542 | VIO5 motY (Rifr Laf− Mot−) | 28 |
| NMB94 | VIO5 motX (Rifr Laf− Mot−) | 26 |
| GRF2 | VIO5 ΔmotY (Rifr Laf− Mot−) | This study |
| E. coli strains | ||
| JM109 | recA1 endA1, gyrA96 thi hsdR17 relA1 supE44 λ− Δ(lac-proAB) F′ traD36 proAB lacIq ΔM15 | 33 |
| BL21(DE3) | Stratagene, San Diego, CA | |
| Plasmids | ||
| pSU38 | kan (Kmr) PlaclacZa | 4 |
| pSU41 | kan (Kmr) PlaclacZa | 4 |
| pIO2 | pSU21; 20-kb SalI fragment (motY+) | 28 |
| pIO6 | pSU38; 1.0-kb HindIII-XbaI fragment (motY+) | 28 |
| pRK101 | pIO6; motY (C147Y) | This study |
| pRK102 | pIO6; motY (R262Q) | This study |
| pRK103 | pIO6; motY (C25Y; −3S of signal sequence to N) | This study |
| pRK201 | pSU38; 1.0-kb HindIII-XbaI fragment (motY C25S) | This study |
| pRK202 | pSU38; 1.0-kb HindIII-XbaI fragment (motY C147S) | This study |
| pRK203 | pSU38; 1.0-kb HindIII-XbaI fragment (motY C25S/C147S) | This study |
| pKS202 | pSU41; 1.0-kb EcoRI-XbaI fragment (6His-motY) | This study |
| pMO401 | pSU41; 700-bp ClaI-BamHI fragment (motX+) | 26 |
| pMO404 | pSU41; 700-bp ClaI-BamHI fragment (motX C35S) | This study |
| pMO405 | pSU41; 700-bp ClaI-BamHI fragment (motX C66S) | This study |
| pMO413 | pSU41; 700-bp ClaI-BamHI fragment (motX C35S/C66S) | This study |
a
Rifr, rifampin resistant; Kmr, kanamycin resistant; Cmr, chloramphenicol resistant; Plac, lac promoter; Pof+, normal polar-flagellar formation; Laf−, defective in lateral-flagellar formation.