TABLE 2.
Swimming fractions
| Host/plasmid | Description | Swimming fraction (%)a |
|---|---|---|
| VIO5/pRK102 | Wild type/MotY-R262Q | 40 (n = 193) |
| GRF2/pIO6 | ΔmotY/wild-type MotY | 61 (n = 208) |
| GRF2/pRK102 | ΔmotY/MotY-R262Q | 29 (n = 192) |
| GRF2/pRK201 | ΔmotY/MotY-C25S | 0 |
| GRF2/pRK202 | ΔmotY/MotY-C147S | <1 |
| GRF2/pRK203 | ΔmotY/MotY-C25S-C147S | <1 |
a
Cells were grown at 30°C for 4 h in VPG broth medium. The swimming cells were counted under a dark-field microscope. The numbers in parentheses indicate the total cells counted. GRF2/pRK201 did not produce any motile cells, whereas GRF2/pRK202 and GRF2/pRK203 produced low but observable numbers of motile cells.