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Gel retardation of the devA promoter by DevA. Results of mobility shift assays in which 3.4 fmol 32P-labeled probe was incubated with 20 ng crude extract from E. coli Rosetta carrying pIJ6973 after induction with IPTG (A) or with 7.5 to 60 nM purified DevA are shown. P, probes alone; C1, control probe (internal to the devA coding sequence) alone; C2, devA promoter probe alone.
