TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or comments | Source or reference |
|---|---|---|
| S. coelicolor strains | ||
| M600 | Prototrophic, SCP1− SCP2− | 32 |
| J3101 | M600 devA::Tn4560 | This work |
| J3102 | M600 devA::Tn5062 | This work |
| J3104 | M600 devB::Tn5062 | This work |
| Plasmids | ||
| pKay1 | Conjugative suicide vector for delivering Tn4560 from E. coli into Streptomyces | 16 |
| pSET152 | Plasmid integrating at phage φC31 attB site, carrying apramycin resistance (apr) | 5 |
| pMS82 | Plasmid integrating at phage φBT1 attB site, carrying hygromycin resistance (hyg) | 22 |
| pIJ6970 | pSET152 carrying 1.5-kb devA fragment (Fig.1) | This work |
| pIJ6972 | pMS82 carrying 1.3-kb devB fragment (Fig 1) | This work |
| pIJ6976 | pMS82 carrying 2-kb devAB fragment (Fig 1) | This work |
| pIJ6979 | pMS82 carrying devAB fragment with in-frame devA deletion (Fig 1) | This work |
| pIJ6973 | pET15b derivative expressing His6-DevA | This work |
| pIJ8660 | egfp-containing vector for transcriptional fusions | 50 |
| pIJ6974 | pIJ8660 derivative expressing a devAp-egfp transcriptional fusion | This work |