FIG. 1.
Effect of hns on the expression of inv. (A) Cultures of MC4100 containing pinv-gfp and VM1303 (an hns mutant) containing pinv-gfp or pinv-gfp and phns were grown overnight in triplicate at 26°C. Fluorescence was calculated by dividing average fluorescence by the OD600. WT refers to MC4100 containing pinv-gfp, hns− refers to VM1303 containing pinv-gfp, and hns−/phns refers to VM1303 containing pinv-gfp and phns. (B) Cultures of JB41v containing the plasmids phns, pymoA (pEll21), or pWKS30strspec were grown overnight in triplicate at 26°C and assayed for expression of inv-phoA. WT refers to JB41v containing pWKS30strspec as a vector control.