FIG. 6.

Ability of H-NS and YmoA to form a repression complex on the inv promoter. (A) Randomly ³²P-labeled inv (−344 to +146) was incubated with purified H-NS and YmoA at room temperature for 15 min and run on a 5% nondenaturing polyacrylamide gel. (B) Randomly ³²P-labeled lacZ was incubated with purified H-NS and YmoA at room temperature for 15 min and run on a 5% nondenaturing polyacrylamide gel. The concentration of YmoA is shown across the top of the gel (in μM), and H-NS was added at a constant concentration of 0.1 μM. The open arrow indicates an H-NS-inv complex, with the closed arrow indicating an H-NS/YmoA-inv complex.