FIG. 7.

Effect of promoter truncations on the expression of inv-gfp in WT and ΔrovA strains. (A) Schematic of the inv promoter with the predicted −35, −10, and +1 sites, along with the start codon (+107) and fusion junction (+170). The predicted RovA binding sites (black lines) from Y. pseudotuberculosis are shown above inv16, and the predicted H-NS binding sites (gray lines) are shown below (21). (B) JB580 (WT) and YVM927 (ΔrovA) strains were transformed with the inv-gfp promoter truncations and grown overnight at 26°C. Fluorescence was calculated by dividing the average fluorescence by OD₆₀₀. V indicates the pROBE-gfp[LVA], containing no promoter, and the numbers below the bars represent the different inv-gfp truncations shown in panel A. Shown below the numbers are the strains in which the truncations were tested.