TABLE 1.

Strains and plasmids

Strain or plasmid	Description	Source
Y. enterocolitica strainsa
JB580c	Serogroup O:8 Nalr ΔyenR(R− M+)	27
JB41v	inv′-′phoA inv+ Nalr Cmr	2
MBL014	inv′-′lacZ inv+ Nalr Cmr	This work
MBL015	ΔrovA inv′-′lacZ inv+ Nalr Cmr	This work
YVM567c	inv′-′phoA inv+ymoB::mTn5Kn2 Nalr Cmr	This work
YVM927c	ΔrovA Cmr	This work
E. coli strains
BL21(DE3)	F−ompT hsdSB(rB− mB−) gal dcm (DE3), a λ prophage carrying the T7 RNA polymerase gene	55
MC4100	F−araD139 Δ(lacIOPZYA)U169 rpsL thiA	14
RO949	MC1000 hns205::Tn10 Tetr	R. Osuna
VM1303	MC4100 hns205::Tn10 Tetr	This work
Plasmids
pBAD33	Expression vector containing PBAD promoter, Cmr	19
pBADrovA:knr	pBAD33 containing Knr cassette in the SphI site and rovA, Cmr	This work
pWKS30:StrSpec	pWKS30 with a Str/Spec cassette in the HindIII site of the polylinker, Ampr Strr Specr	Lab collection
pELL21	pWKS30::StrSpec carrying ΔymoBymoA and upstream promoter region	12
pET-24+	Overexpression vector with T7 promoter and C-terminal His tag	Novagen
pETrovA-his	RBS plus rovA open reading frame cloned into pET	This work
pEThns-his	RBS plus hns open reading frame cloned into pET	This work
PGEX-6P-1	Overexpression vector with tac promoter and cleavable N-terminal GST tag	Pharmacia
pGST-ymoA	ymoA open reading frame cloned into pGex	This work
phns	pWKS30::StrSpec carrying hns and upstream promoter region	This work
pROBE-gfp[LVA]	gfp with LVA tag to decrease its half-life, Knr	39
pinv16	pROBE-gfp[LVA], containing inv promoter fragment −305 to +146	This work
pinv29	pROBE-gfp[LVA], containing inv promoter fragment −276 to +146	This work
pinv30	pROBE-gfp[LVA], containing inv promoter fragment −239 to +146	This work
pinv31	pROBE-gfp[LVA], containing inv promoter fragment −190 to +146	This work
pinv17	pROBE-gfp[LVA], containing inv promoter fragment −158 to +146	This work
pinv12	pROBE-gfp[LVA], containing inv promoter fragment −69 to +146	This work

^aAll Y. enterocolitica strains listed are derivatives of JB580v.