FIG. 1.

Competence-dependent expression of CoiA. (A) Kinetics of competence development of CoiA-His₆ mutant in CP1824 (⧫) and transcriptional profile of coiA in CP1250 (▪). (B) CoiA-His₆ protein levels were determined in strain CP1824 in two replicate experiments. (C) Comparison of the transformation phenotype between CP1824 and CP1250. Details for the transformation assay and immunoblot analysis can be found in the Materials and Methods. Details for the coiA mRNA can be found in the report from Peterson et al. (46).