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Competence development in the presence of overexpressed CoiA. (A) Transformation of CP1874 was determined at 2.5-min intervals after adding CSP-1 to a culture grown for 1 generation in 100 μM raffinose. (B) CoiA-His6 protein levels determined by immunoblot analysis in extracts prepared at 5-min intervals. (C) Transformation phenotypes of CP1874 and CP1250, respectively.
