TABLE 5.
DNA uptake and processing in coiA mutant
Strain | DNA (cpm ml−1) at processing stagea:
|
Transformation (Novr cells ml−1) | ||
---|---|---|---|---|
Degradationb | Bindingc | Uptaked | ||
CP1250 (wild type) | 1,060 ± 37 | 1,140 ± 30 | 600 ± 27 | 1,900 ± 450 |
CP1793 (coiA null) | 880 ± 88 | 900 ± 61 | 440 ± 4 | 53 ± 30 |
CP1389 (dprA null) | 990 ± 38 | 890 ± 99 | 600 ± 30 | 0.0001 |
Cells were exposed to CSP and 105 cpm DNA for 30 min at 30°C. Data are means ± standard errors of results from three independent experiments.
Acid-soluble extracellular products.
DNase-susceptible bound DNA.
DNase-resistant bound DNA.