FIG. 3.

Verification of the function of DsbD^TH. (A) Scheme showing DsbD^TH, DsbD containing the thrombin cleavage site between the domains, and the c-Myc epitope after the thrombin cleavage site between the α and β domains. (B) The in vivo redox states of DsbC were assessed in the strains expressing wild-type DsbD, wild-type DsbD^TH, and P284A DsbD^TH using AMS alkylation. In lane 1, SEN45 (the dsbD mutant) containing only the vector (pAM238) was used. In the other lanes, the cells express wild-type DsbD (pSC01, lanes 2 to 4), wild-type DsbD^TH (pSC49, lanes 5 to 7), and P284A DsbD^TH (pSC49-10, lanes 8 to 10).