FIG. 1.

Inhibition of growth and ATP hydrolysis activity of inverted membrane vesicles by DCCD. (A) Growth of C. paradoxum in the presence of 50 μM DCCD (▵) or tetrachlorosalicylanilide (20 μM) or an equivalent volume of ethanol (•). The arrow indicates addition of inhibitor or ethanol at mid-exponential growth phase. (B) Inverted membrane vesicles (100 μg of protein) were incubated in 0.1 ml of buffer containing 50 mM MOPS-KOH, pH 7.5, at 25°C in the presence of 200 μM DCCD (▵) or an equal volume of absolute ethanol (•). Samples (20 μl) were taken at the times indicated, and the amount of ATPase activity was determined via the ATP-regenerating assay.