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Stimulation of the C. paradoxum F1Fo-ATPase by NaCl. The ATPase was dialyzed overnight as described in Materials and Methods. Purified ATPase (10 μg) was used to determine the effect of increasing concentrations of Na+ ions on ATPase activity. The ATPase assay mixture contained either 50 mM MOPS (pH 7.0) (•) or 50 mM Tricine-KOH (pH 9.0) (▵), and the NaCl concentrations indicated.
