FIG. 1.
Bio-Gel P4 chromatography of cyclic β-1,2-glucans accumulated in vivo by wild-type and mutant strains. Cells from 100-ml cultures were harvested and extracted with 70% ethanol. The extracts were subjected to chromatography on Bio-Gel P4 columns (78 by 1.8 cm). The glucans eluted with 0.1 M pyridine-acetate buffer (pH 5.5). Fractions of 1.5 ml were collected, and carbohydrates were expressed as μmol glucose equivalents (g wet weight)−1. (A) B. abortus 2308 cellular glucans. (B) B. abortus 2308 cellular glucans after mild alkali treatment (0.1 N NaOH for 30 min at 37°C). (C) B. abortus Cgm08 cellular glucans. (D) B. abortus Cgm(pBB4cgm) cellular glucans. V0, void volume; Vt, total volume; I, anionic glucan; II, neutral glucan.