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. 2006 Jul;188(14):5003–5013. doi: 10.1128/JB.00086-06

FIG. 2.

FIG. 2.

DEAE-Sephadex chromatography of cellular glucans accumulated by wild-type and mutant strains. Glucans were recovered from Bio-Gel P4 columns (fractions 13 to 22) and subjected to chromatography on DEAE-Sephadex columns (0.25 by 3 cm). The glucans were eluted with 1.5 ml water (fractions 1 to 3), 1.5 ml 0.05 M NaCl (fractions 4 to 6), 1.5 ml 0.1 M NaCl (fractions 7 to 9), 1.5 ml 0.25 M NaCl (fractions 10 to 12), and 0.5 M NaCl (fractions 13 to 15). Fractions of 0.5 ml were collected, and carbohydrates were measured by the anthrone-sulfuric acid method (20). (A) B. abortus 2308 cellular glucans. (B) B. abortus 2308 cellular glucans after mild alkali treatment (0.1 N NaOH for 30 min at 37°C). (C) B. abortus Cgm08 cellular glucans. (D) B. abortus Cgm08(pBB4cgm) cellular glucans.